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crx gfp  (TaKaRa)


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    TaKaRa crx gfp
    Crx Gfp, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 89 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/crx+gfp/CRE+Recombinase+Gesicles/pm39996725-86-7-21
    Average 95 stars, based on 89 article reviews
    crx gfp - by Bioz Stars, 2026-10
    95/100 stars

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    Article Title: Generation of a Double Reporter mES Cell Line to Simultaneously Trace the Generation of Retinal Progenitors and Photoreceptors.
    Article Snippet: .. The floxed copGFP-Puro selection cassette from the Crx-GFP;Rax-mCherry cell line clone 1–2 was removed by transfection with Cre recombinase-containing gesicles (631449, TaKaRa, Kusatsu, Japan). .. GFP-negative cells were sorted at the Flow Cytometry Facility (FCF) of the University of Lausanne (UNIL) with FACS sorter Beckman Coulter MoFlo Astrios EQ (Beckman Coulter, Brea, CA, USA) and seeded into 3 96-well plates for single cell propagation.

    Article Title: Generation of a Double Reporter mES Cell Line to Simultaneously Trace the Generation of Retinal Progenitors and Photoreceptors
    Article Snippet: .. The floxed copGFP-Puro selection cassette from the Crx-GFP;Rax-mCherry cell line clone 1–2 was removed by transfection with Cre recombinase-containing gesicles (631449, TaKaRa, Kusatsu, Japan). .. GFP-negative cells were sorted at the Flow Cytometry Facility (FCF) of the University of Lausanne (UNIL) with FACS sorter Beckman Coulter MoFlo Astrios EQ (Beckman Coulter, Brea, CA, USA) and seeded into 3 96-well plates for single cell propagation.

    Transfection:

    Article Title: Generation of a Double Reporter mES Cell Line to Simultaneously Trace the Generation of Retinal Progenitors and Photoreceptors.
    Article Snippet: .. The floxed copGFP-Puro selection cassette from the Crx-GFP;Rax-mCherry cell line clone 1–2 was removed by transfection with Cre recombinase-containing gesicles (631449, TaKaRa, Kusatsu, Japan). .. GFP-negative cells were sorted at the Flow Cytometry Facility (FCF) of the University of Lausanne (UNIL) with FACS sorter Beckman Coulter MoFlo Astrios EQ (Beckman Coulter, Brea, CA, USA) and seeded into 3 96-well plates for single cell propagation.

    Article Title: Generation of a Double Reporter mES Cell Line to Simultaneously Trace the Generation of Retinal Progenitors and Photoreceptors
    Article Snippet: .. The floxed copGFP-Puro selection cassette from the Crx-GFP;Rax-mCherry cell line clone 1–2 was removed by transfection with Cre recombinase-containing gesicles (631449, TaKaRa, Kusatsu, Japan). .. GFP-negative cells were sorted at the Flow Cytometry Facility (FCF) of the University of Lausanne (UNIL) with FACS sorter Beckman Coulter MoFlo Astrios EQ (Beckman Coulter, Brea, CA, USA) and seeded into 3 96-well plates for single cell propagation.



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    Image Search Results


    Generation of hESC derived retina organoids (CRX-GFP cell line) and RPE (H9 cell line). (A) Phase contrast images of organoid development up to day 56. (B–D) Progenitor gene expression analysis of CRX-GFP hESC-derived retina organoids ( n = 2) and undifferentiated stem cells ( n = 1). (B) OCT4 expression is absent in differentiated day 48 retina organoids. (C) Retina organoids (red) highly express genes indicative of primitive retina and photoreceptor progenitors. (D) Markers for mature, terminally differentiated retinal subpopulations. Retina organoids (red) contain markers for all the major retinal cell types, including photoreceptor progenitors, glia, ganglion cells, bipolar, horizontal, amacrine, and Müller cells. (E) Flow analysis shows that GFP expression is absent in the undifferentiated stem cells, early embryoid bodies, and young retina organoids. A distinct GFP+ population appears at day 70. Expression is highest day 78 and persists in the oldest analyzed day 91 organoids. (F) Examples of gates drawn used for FACS sorting of the GFP negative and GFP positive populations. (G) Post-FACS purities >90%. Cells were gated based on singlets, SSC vs. FSC profile, and if they were DAPI negative. Negative controls were undifferentiated CRX-GFP hESC (day 0). (H) Parylene only implant (no cells). (I) CPCB-RPE1 implant covered with hESC-RPE cells. (J) Enlargement of (I) to show details of hESC-RPE cells forming an epithelium (28–35 days culture).

    Journal: Frontiers in Neuroscience

    Article Title: Co-grafts of Human Embryonic Stem Cell Derived Retina Organoids and Retinal Pigment Epithelium for Retinal Reconstruction in Immunodeficient Retinal Degenerate Royal College of Surgeons Rats

    doi: 10.3389/fnins.2021.752958

    Figure Lengend Snippet: Generation of hESC derived retina organoids (CRX-GFP cell line) and RPE (H9 cell line). (A) Phase contrast images of organoid development up to day 56. (B–D) Progenitor gene expression analysis of CRX-GFP hESC-derived retina organoids ( n = 2) and undifferentiated stem cells ( n = 1). (B) OCT4 expression is absent in differentiated day 48 retina organoids. (C) Retina organoids (red) highly express genes indicative of primitive retina and photoreceptor progenitors. (D) Markers for mature, terminally differentiated retinal subpopulations. Retina organoids (red) contain markers for all the major retinal cell types, including photoreceptor progenitors, glia, ganglion cells, bipolar, horizontal, amacrine, and Müller cells. (E) Flow analysis shows that GFP expression is absent in the undifferentiated stem cells, early embryoid bodies, and young retina organoids. A distinct GFP+ population appears at day 70. Expression is highest day 78 and persists in the oldest analyzed day 91 organoids. (F) Examples of gates drawn used for FACS sorting of the GFP negative and GFP positive populations. (G) Post-FACS purities >90%. Cells were gated based on singlets, SSC vs. FSC profile, and if they were DAPI negative. Negative controls were undifferentiated CRX-GFP hESC (day 0). (H) Parylene only implant (no cells). (I) CPCB-RPE1 implant covered with hESC-RPE cells. (J) Enlargement of (I) to show details of hESC-RPE cells forming an epithelium (28–35 days culture).

    Article Snippet: The first one, the cone-rod homeobox gene-green fluorescent protein (CRX-GFP) hESC line , was maintained with TeSR E8 media on Vitronectin XF (Stemcell Technologies) coated plates.

    Techniques: Derivative Assay, Gene Expression, Expressing

    Transplant histology at 2 months (A,B) and 6.5 months (C–E) post-surgery. (A,C) Co-graft area. (B,D) Host retina outside co-graft. (A–D) H&E staining. White asterisks indicate the center of rosettes (spherical arrangement of photoreceptors and other retinal layers around a central lumen). The transplant in (C) has grown around the parylene RPE implant (transplant #3 in , 197 dps). (E) Bestrophin (gold) and DAPI (blue) shows RPE and parylene in transplant (#2 in , 195 dps). Blue arrows point to parylene. Red arrows point to hESC-RPE. Yellow arrows point to host RPE. The separation of tissue is processing artifact. Scale bars = 100 μm (A–D) ; 20 μm (E) .

    Journal: Frontiers in Neuroscience

    Article Title: Co-grafts of Human Embryonic Stem Cell Derived Retina Organoids and Retinal Pigment Epithelium for Retinal Reconstruction in Immunodeficient Retinal Degenerate Royal College of Surgeons Rats

    doi: 10.3389/fnins.2021.752958

    Figure Lengend Snippet: Transplant histology at 2 months (A,B) and 6.5 months (C–E) post-surgery. (A,C) Co-graft area. (B,D) Host retina outside co-graft. (A–D) H&E staining. White asterisks indicate the center of rosettes (spherical arrangement of photoreceptors and other retinal layers around a central lumen). The transplant in (C) has grown around the parylene RPE implant (transplant #3 in , 197 dps). (E) Bestrophin (gold) and DAPI (blue) shows RPE and parylene in transplant (#2 in , 195 dps). Blue arrows point to parylene. Red arrows point to hESC-RPE. Yellow arrows point to host RPE. The separation of tissue is processing artifact. Scale bars = 100 μm (A–D) ; 20 μm (E) .

    Article Snippet: The first one, the cone-rod homeobox gene-green fluorescent protein (CRX-GFP) hESC line , was maintained with TeSR E8 media on Vitronectin XF (Stemcell Technologies) coated plates.

    Techniques: Staining